Bees can learn to associate colors with a sugar reward in a setting closely resembling a natural foraging situation
This artificial flower patch consists of 24 Eppendorf tubes (4 cm deep) that function as flowers
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They are regularly distributed over the surface of a foraging arena. This arena consists of two superposed acrylic plates, creating 24 holes of 1 cm in diameter
The lower plate is created with a 0.7 cm thick opaque acrylic plastic, while the upper square was a 0.2 cm thick transparent Plexiglas
The tubes are placed inside the holes and raised 1.8 cm above the upper surface of the transparent Plexiglas
24 color signals, 12 yellow and 12 blue, can be are visible to the bees through the upper transparent Plexiglas plate
The flowers were held by the upper surface of the patch and the colored circles were set below this surface, both the flowers and their corresponding visual stimuli could easily be replaced between trials.
