The spectral characteristics of the dyes used for the dual colour encoded system are:
Color 1:
Excitation 420 – 480 nm
Color 2:
Excitation 515 – 540 nm
Emission 485 – 540 nm
Emission 535 – 570 nm
Dual color encoded have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software.